Plasmid

Part:BBa_K243037:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)

pBAD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2434
    Illegal SpeI site found at 3052
    Illegal PstI site found at 3066
    Illegal NotI site found at 2440
    Illegal NotI site found at 3059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2434
    Illegal BamHI site found at 2338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3052
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3106
    Illegal BsaI site found at 3284
    Illegal BsaI site found at 4352
    Illegal SapI site found at 2155
    Illegal SapI.rc site found at 783
    Illegal SapI.rc site found at 2949


Design Notes

Commented GenBank file

Source

Created by several modifications on the pBAD vector from invitrogen.

References

Guzman, L.-M., Belin, D., Carson, M. J., and Beckwith, J. (1995). Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter. J. Bacteriol. 177, 4121-4130.